Mutation effect predictor: what does a DNA change do to the protein?
Paste a coding DNA sequence and type one or more changes in HGVS notation, such as c.35G>A. The mutation effect predictor applies each change to the sequence, translates the result and reports the protein change, the type of consequence (missense, frameshift, start lost and so on), an impact class, and for single amino acid changes the Grantham distance and BLOSUM62 score.
What you get
- The protein-level change, such as p.Gly12Asp, with the old and new codon
- The consequence: missense, frameshift, start lost and others
- An impact class (for example MODERATE or HIGH)
- Grantham distance with its class, and the BLOSUM62 score
- Up to 20 changes in one run
How to use it
- Open the Mutate tab and paste a coding DNA sequence.
- Type the changes, one per line (for example
c.35G>A). - Choose the first ATG or position 1 as the start, and run it.
Worked example
Click KRAS hotspots in the Mutate tab, or use the KRAS coding sequence with these changes:
| Change | Result | Grantham / BLOSUM62 |
|---|---|---|
c.35G>A | p.Gly12Asp (GGT→GAT): missense, impact MODERATE | 94 (moderately conservative) / −1 |
c.34G>T | p.Gly12Cys (GGT→TGT): missense, impact MODERATE | 158 (radical) / −3 |
c.1A>G | p.Met1?: start lost, impact HIGH | not applicable |
c.35del | p.Gly12ValfsTer3: frameshift, impact HIGH | not applicable |
Limits to know about
- The sequence must be plain DNA (A, C, G, T), up to 1,000,000 bases, and up to 20 changes at a time.
- It reports the effect on the protein sequence. It does not tell you whether a variant causes disease, and it is not medical advice.
Common questions
What does c.35G>A mean?
The 35th base of the coding sequence changes from G to A. The variant effects guide explains HGVS notation and the consequence types.
What are Grantham and BLOSUM62?
Two ways to score how different the new amino acid is from the old one. A higher Grantham distance means a bigger chemical change; a lower BLOSUM62 score means the swap is rarer among related proteins.
Can I use this to decide whether a variant is harmful?
No. It predicts a change to the protein sequence only. Clinical interpretation needs experts and databases; see the terms.
Are my sequences stored?
No. Sequences are processed in memory to produce your result and are not stored or logged. The privacy policy has the details, including what BLAST sends to NCBI and EMBL-EBI.