Free DNA, RNA and protein analysis tools
Every tool runs in your browser session on the server, needs no account, shows no advertising and does not store your sequences. Each page below explains what the tool does, its limits and a worked example, and opens the tool itself.
GC content calculator for DNA and RNAGC and AT percent, base counts, a sliding-window plot and GC skew.ORF finderATG-to-stop ORFs on both strands, longest first, with translations.Restriction site finder and restriction mapperPositions and counts for 36 common restriction enzymes.PCR primer design toolEight ranked primer pairs with Tm, GC, hairpin and dimer checks.Pairwise sequence alignment toolLocal or global alignment of two sequences, with score and identity.Phylogenetic tree maker (UPGMA)UPGMA tree from 2–100 FASTA sequences, with Newick output.CRISPR guide RNA finderScored guides for SpCas9, SpCas9-NG, SaCas9 and Cas12a.Mutation effect predictorProtein change, consequence, impact, Grantham and BLOSUM62 for HGVS changes.Protein property calculatorMolecular weight, pI, GRAVY, instability, extinction coefficient and more.
New to a topic? The guides explain the ideas in plain language.